Global assembling of Academicians, Researchers, Scholars & Industry to disseminate and exchange information at 100+ Allied Academics Conferences
Gabriela Hr?kova graduated from the University of P. J. Šafárik, Faculty of Sciences in Košice, (Slovakia), where she completed Master’s degree in biology. She achieved PhD degree in 1993 at the Institute of Parasitology, Slovak Academy of Sciences and became a staff member. Her present position is principal investigator and Head of laboratory of Experimental Pharmacology at this Institute. Since 1997 she participates in pre-graduate and post graduate education both as supervisor and lecturer at Faculty of Sciences, Šafárik University. Since 2009 she also works for private Pharmaceutical Company (ImmunaPharm) in Slovakia as consultant for molecular and cell biology. Her research has been awarded by grants from the Royal Society of UK, Wellcome Trust London, ÖAD Austria, EU “Synthesis” and by several fellowships. She is author or co-author of over 60 scientific papers, 3 book chapters and one book published in series SpringerBrief, which are indexed in WOS/Scopus. Her background in pharmacology of infectious diseases extends over more than 25 years of research. Main research interests cover the effects of anthelmintic drugs and natural compounds, drug carriers and immunomodulators using in vitro and in vivo experimental settings, as well as interests in mechanisms of host-parasites interactions.
Larval stage of flatworm, tetrathyridium, has ability of survival and asexual multiplication in a wide range of vertebrate hosts what indicates a high biochemical and physiological potential for adaptation. We recently developed axenic long-term in vitro cultivation systems of larvae under both hypoxic and aerobic conditions, representing the unique eukaryotic model for pharmacological and molecular studies. This in vitro system allowed us to study the dose- and time-dependent effects of various natural compounds on multiple biochemical and molecular pathways in larvae. We have focussed on flavonolignans (silybin, dehydrosilybin and silychristin) prepared from silymarin, the main component of herb Silybum marrianum and two other flavonoids: bergenin and arbutin. Activity of individual compounds on metabolic activity of larvae, which reflects activity of enzymes of complex I and II in mitochondria, was dependent on oxygen tension in culture. Flavonolignans also modulated activity of other enzymes like GST, SOD, enzymes involved in glucose transport, in lipogenesis, cell death and motility, indicating their complex activity on multiple targets in larvae.